Autism Spectrum Disorder (ASD) is a range of neurodevelopmental disorders resulting in communication, learning, and behavioral deficiencies. Interestingly, human patients with ASD have altered synaptic density and microglia activation. Through a collaboration with the Kampmann lab at UCSF, we have an interest in investigating the functional role of ASD-risk genes identified from a CRISRRi-based screening from Teter et al., 2024 (Biorxiv). Using CRISPR mutants combined flow cytometer-based engulfment assay and scRNA sequencing technologies, we hope to gain a basic understanding of microglia-synaptic interactions in models of ASD.